The present paper deals with In vitro study of Agave americana L. The activity was carried out by using acetone and ethyl acetate as solvents. The extraction was made by Soxhlet’s apparatus and agar well diffusion method was followed. A maximum inhibition zone (20mm) against E.coli was recorded in ethyl acetate solvent. While, a zone of (21 mm) was observed against E.coli in acetone extract. Thereby it is proved that Agave americana L. possess a congenial anti-bacterial activity in both solvents.