Traditionally, wheatgrass has been used since ages for treating a number of diseases. There is no systematic experiment has been carried out on the effect of Wheat grass juice on ISO-induced myocardial ischemia. Therefore, the present study is to examine whether Wheat grass juice will attenuate the progression of myocardial ischemia induced by iso proterenol. The in vitro study shows, increased intracellular ROS levels were observed in ISO-induced H9C2 cells as evidenced by increased DCF florescence. Whereas WG (10 µM) treatment prior to ISO-induction significantly reduced intracellular ROS levels, this is due to the possible scavenging of ROS during ISO-induced cells. Based on our results and along with previous reports we state that the WG could scavenge ISO-induced free radicals. We also examined the involvement of mitochondrial damage in ISO-induced apoptosis. To investigate the effect on mitochondria, we performed Rh-123 staining in H9C2 cells. The present findings showed that ISO-induced loss of Δψm have been restored by WG pretreatment. It suggests that WG protects cells from apoptosis by inhibition of the mitochondrial alteration.