Andrographis paniculata is an herbaceous plant of the family of Acanthaceae, found in tropical and subtropical regions and has wide application in herbal medicine preparation. Stem and leaf and even whole plant are to cure various ailments in human beings. In fact, plants produce a diverge range of bioactive principles, making them a rich source of different types of medicines researches. Bioactive substances lead to the discovery of new compounds that could be used to formulate new and most potent antimicrobial drugs to overcome the problem of resistant to the currently available medicines. Acetone, alcohol, benzene, chloroform, and water extracts of leaf and stem were used for phytochemical screening and antimicrobial activity.