Background: The pH of carbonated drinks is known to be low and have, therefore, been implicated in the increasing incidence of acidity.Aim: Here we report on an efficient approach for rapid analysis of nucleic acid characterization by varying a cost-effective running buffers for agarose gel electrophoresis.Material and Methods: Commercial DNA is used in electrophoretic analysis using various soft beverages by optimizing its pH value and gel bands were quantified using Image.Results: Red bull energy drink revealed that concentration, time and voltage do not have any effect in analysis of nucleic acids, while fizz revealed high concentration and sensitivity in characterization of nucleic acids.Conclusion: This approach was fast, easy and cost-effective than use of TAE or TBE as running buffers in agarose gel electrophoresis.