Barleria gibsoni Dalz. (Acanthaceae) is commonly known as Neel Koranti, and traditionally used in cataract, ulcer and fever. The Present study was carried out to investigate antioxidant potential of ehanolic and aqueous extracts of Barleria gibsoni, by DPPH, Nitric oxide and hydroxyl radical scavenging activity. The DPPH radical inhibition (%) was 85.79, 66.34 and 71.33 for BGLE, BGLA and ascorbic acid respectively. The Barleria gibsoni extracts (BGLE and BGLA) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 1000μg/ml, showing 71.19% and 61.22% of NO inhibition, respectively. The BGLE and BGLA extracts significantly scavenged the hydroxyl radical generated by the EDTA/H₂O₂ system, when compared to that of ascorbic acid.