International Journal of Biology Research

International Journal of Biology Research


International Journal of Biology Research
International Journal of Biology Research
Vol. 6, Issue 2 (2021)

Evaluation of in Vitro phagocytic property of macrophages in presence of thymopure™ (Nigella Sativa) oil


S Mehkri, K Chandrasagar, G Ashok, Krathish Bopanna

Black cumin or Nigella sativa (N. sativa) has been used for its medicinal properties for centuries in many parts of the world. Studies have shown that Nigella sativa helps reinforce the body's defence responses through antioxidant and anti-inflammatory properties. Thymoquinone, a benzoquinone compound which is the main active component, exhibits anti-inflammatory activity through Toll-Like Receptor 4 (TLR-4) and is able to increase the activity of macrophages against invading foreign bodies. Mouse macrophages which mimic natural macrophages were used in the current study to investigate the benefits of ThymoPureTM (N. sativa) oil on potentiation of phagocytosis. ThymoPureTM (N. sativa) oil was initially studied for its in vitro cytotoxicity, by exposing the cells to different concentrations of the test substance. Non-toxic concentrations of 125 µg/ml and 62.5 µg/ml were identified and selected for the phagocytosis study. Macrophage (Raw 264.7) cells were treated with the test substance for 1 h then exposed to green E.coli slurry. The amount of phagocytosis observed in macrophage cells was estimated by fluorescent microscopy and flow cytometry. ThymoPureTM (Nigella Sativa) oil increased the phagocytic capacity of cells compared to the cell control. Further, flow cytometry analysis showed the increase in phagocytic capacity of the cells in both lower (18.7%) and higher concentrations (39.1%) of the test substance. ThymoPureTM (N. sativa) oil has shown to have some potentiation effects on phagocytosis on mouse macrophages in our study.
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How to cite this article:
S Mehkri, K Chandrasagar, G Ashok, Krathish Bopanna. Evaluation of in Vitro phagocytic property of macrophages in presence of thymopure™ (Nigella Sativa) oil. International Journal of Biology Research, Volume 6, Issue 2, 2021, Pages 18-21
International Journal of Biology Research