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VOL. 6, ISSUE 2 (2021)
Evaluation of in vitro immuno modulatory activity of thymopure™ Nigella sativa oil in dendritic cells
Authors
S Mehkri, K Chandrasagar, G Ashok, Krathish Bopanna
Abstract
Nigella sativa or black seed has been reviewed in greater detail in the past decade, for its role on immunomodulatory and immunotherapeutic potential. Studies have shown it to have potential anti-inflammatory effects on several inflammation-based models. The current study evaluated the effects of Thymo Pure TM (Nigella sativa) oil on LPS-induced dendritic cells maturation, survival and cytokine release. Thymo Pure TM (Nigella sativa) oil was initially examined for cytotoxicity against dendritic cells, in vitro by MTT assay. The dendritic cells were exposed to different concentrations of the test substance. Non-toxic concentrations were identified and were used for further efficacy studies. Thymo Pure TM (Nigella sativa) oil inhibited LPS induced TNF-α in dendritic cells by 47.75% ± 1.6% and 38.77% ± 0.9%, IL-1- β levels by 40.60% ± 2.70% and 36.36% ± 3.08%, IL-6 by 53.48% ± 2.1% and 26.24% ± 1.6% and IL-17 by 43.98% ± 2.1% and 21.45% ± 2.6%, over the control at test doses of 125 µg/ml and 62.5 µg/ml, respectively. Thymo Pure TM (Nigella sativa) oil counteracts DC maturation. Upregulation of several maturation markers like TNF-a, IL-6, IL-1beta and IL-17 following LPS exposure, was inhibited in the presence of ThymoPureTM (Nigella sativa) oil in the current study, which further substantiates its immuno modulatory activity.
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Pages:53-57
How to cite this article:
S Mehkri, K Chandrasagar, G Ashok, Krathish Bopanna "Evaluation of <em>in vitro </em>immuno modulatory activity of thymopure™ <em>Nigella sativa </em>oil in dendritic cells ". International Journal of Biology Research, Vol 6, Issue 2, 2021, Pages 53-57
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