Evaluation of thymopureTM (Nigella sativa) oil on the in vitro expression levels of selected cytokines in mouse macrophages cells
S Mehkri, K Chandrasagar, G Ashok, Krathish Bopanna
Nigella Sativa has been shown to exhibit anti-inflammatory and anti-oxidant effects on cells in literature. Increased concentrations of TNF-α, COX-2, iNOS are found in acute and chronic inflammatory conditions. These mediators have been shown to play a key role in inflammatory process and thus can be a potential target for therapeutic intervention. The current study was designed to evaluate the effects of ThymoPureTM (Nigella sativa) oil on the in-vitro expression level of cytokines TNF-α, COX-2 and iNOS in mouse macrophage cells. ThymoPure™ (Nigella sativa) oil was initially studied for cytotoxicity on mouse macrophage cells by exposing them to different concentrations of the test substance. Further two non-toxic doses of 125 mg/ml and 62.5 mg/ml were selected for the study. Mouse macrophage cells were treated with 125 mg/ml and 62.5 mg/ml of the test substance along with LPS (1 µg/ml) and were incubated for 24 hours. The cells were then harvested for studying the gene expression level of TNF-α, COX-2 and iNOS by Quantitative RT-PCR analysis. The study results showed a decrease in mRNA gene expression of TNF-α (0.97 & 1.17 folds), iNOS (0.91 & 0.99 folds) and COX-2 (1.05 & 1.17 folds) on exposure to lower and higher dose of the test substance respectively when compared to the cell control. The level of expression was observed to decrease when the cells were treated with higher dose (125 μg/ml) in comparison to lower dose (62.5 μg/ml) of ThymoPureTM (Nigella sativa) oil for 24 hours. In the current in-vitro study ThymoPureTM (Nigella sativa) oil has shown potential anti-inflammatory effects as it reduced the expression of TNF-α, COX-2 and iNOS in an accelerated dose response.
S Mehkri, K Chandrasagar, G Ashok, Krathish Bopanna. Evaluation of thymopureTM (Nigella sativa) oil on the in vitro expression levels of selected cytokines in mouse macrophages cells. International Journal of Biology Research, Volume 6, Issue 4, 2021, Pages 5-10