Natural carotenoids are secondary metabolites with antioxidant, anti-inflammatory, and cancer-preventative activities. The pharmaceutical, cosmetic, textile, and food industries all have a high need for these molecules, prompting the hunt for alternative natural sources of carotenoids. Carotenoids are light, heat, oxygen, acids, and alkaline bases sensitive. When exposed to light (direct sunlight/UltraViolet), cis–trans photoisomerization occurs, which may result in photodestruction. Biological materials that include carotenoids, as well as their solutions, must be protected from light. Many carotenoids (xanthophylls) are thermolabile, hence they should only be heated when absolutely necessary. The generation of carotenoids from bacteria has sparked a lot of attention in the industrial world in recent years. Some bacteria can manufacture distinctive carotenoids with C30 skeletons in addition to carotenoids with C40 skeletons. This article discusses the isolation and screening of bacteria that produce carotenoids. After primary, secondary, tertiary, and quaternary screening of isolates based on pigmentation, tolerance to pH and NaCl percent (w/v), degree of pigmentation, absorption maxima, free radicle scavenging activities, and it’s antimicrobial activity, effective carotenoid producers were identified. In this regard, a wide range of methods for extracting and identifying bacterial carotenoids have been published, and this is the review that summarises most of this knowledge.We present their biosynthetic origin to focus on the strategies used in their extraction and characterisation in order to comprehend the diversity of these carotenoids. For the analysis and identification of bacterial carotenoids, high-performance liquid chromatography-mass spectrometry (HPLC-MS) has received special attention. We'll wrap off this section by discussing the commercial possibilities of bacterial carotenoids. This review is intended to serve as a reference for identifying these metabolites, which are regularly found in new bacterial strains.