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International Journal of
Biology Research
ARCHIVES
VOL. 7, ISSUE 3 (2022)
Assessment of direct wet mount and various concentration approaches for diagnosing of Entamoeba histolytica /dispar and Giardia lamblia cysts in human stool samples
Authors
Hiro Mohammed Obaid
Abstract
The human gastro-intestinal tract is preferred by the parasites Entamoeba histolytica/dispar and Giardia lamblia. As a result, stool samples are the primary way that their stages are found. The current study's objective was to assess several approaches for locating E. histolytica/dispar and G. lamblia cysts in human stool samples. Direct wet mount and concentration procedures were used to analyse all feces samples. Both flotation and formal ether sedimentation were used as concentration techniques. Entamoeba and Giardia had significant prevalence rates among the Kirkuk population, with 13.7 percent and 5.1 percent, respectively. Additionally, according to the findings of this study, children from 1 month to 2 years old were the most frequently affected age group by both parasites. No differences between males and females were discovered. Both direct and concentration methods were utilized in this experiment to identify the cystic stage. The number of cases were actually found differed depending on the methodology that we applied. The cyst count was higher employing flotation and sedimentation techniques, and it was considerably higher when using saturated salt solutions and zinc sulfate for the flotation process. We came to the conclusion that the direct wet mount approach is less efficient than the concentration method; it is therefore preferable to employ the concentration method, particularly when the parasite population is low and the diagnosis is in doubt. We advise including concentration techniques based on several concepts in routine diagnostic testing in order to increase the sensitivity of the direct test.
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Pages:17-21
How to cite this article:
Hiro Mohammed Obaid "Assessment of direct wet mount and various concentration approaches for diagnosing of <em>Entamoeba histolytica</em> /<em>dispar</em> and <em>Giardia lamblia</em> cysts in human stool samples". International Journal of Biology Research, Vol 7, Issue 3, 2022, Pages 17-21
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