This study probes the exploitation of some agricultural wastes and forestry plants for the growth of Pleurotus ostreatus. Mycelium of this mushroom species was cultivated on sterile, dried and crushed leaves of wheat straw, Typha latifolia L. and Ampelodesmos mauritanicus (Poir), olive pomace, and coffee and tea wastes with different particle sizes. Daily elongation of oyster mushroom colony was conducted in Petri dishes. Statistical analyses demonstrated a faster colonization of A. mauritanicus and T. latifolia media and that substrates particle size between 1-2 mm was the highest granulometric size for oyster elongation. Properties on mineral composition, pH, water retention capacity, lignin, and cellulose were identified for all used media. Principal components analysis, according physicochemical parameters, showed that T. latifolia appears very distinguishable and discarded from others substrates. Water capacity retention was appeared the most discriminating and distinctive parameter between all physical and mineral ones for the oyster hyphal elongation.