Harmful bacteria strain of E.coli could be present in public drinking water sources. It has become an unignorable public health concern across the world, such as water borne epidemic outbreak causes severe diarrhoea in human any many more. The study determine and identify the prevalence of E.coli in public drinking water sources in the state Meghalaya through morphological and cultural characteristic, using standard microbial cultural technique. In order to improve community sanitation and hygiene practices. Out of 200 water samples analyse, 58 samples showed the present of E.coli, obtaining the overall, prevalence of 29%. 24 samples (12%) were observe from raw water, 16 samples (8%) from tap water, 10 samples (5%) from health center water, 5 samples (2.5%) from treated water, and 3 samples (1.5%) from packaged drinking water. The study concludes with a firm recommendation to firstly treat, boil, and filter the raw and tap water before consumption or it use in daily washing of fruits and vegetables to avoid any possibility of water borne outbreak.