Pigments are natural as well as manmade biochemical compounds produced by plants and microorganisms which exhibits specific colors. [1] They are mostly used in dye and textile industries, cosmetics and also in medicines due to its antimicrobial abilities. [1] Serratia marcescens belongs to a group of pigmented bacteria which produces a pigment called as “Prodigiosin” – a secondary metabolite. In this study, the bacteria was isolated from various samples (soil, water, coconut) on nutrient agar supplemented with glycerol which act as growth enhancers for the bacteria. Strains which shows red pigmentation were selected. Various morphological and biochemical tests were performed for identification of the strain. Extraction of the pigment was carried out using plate-swabbing methanol method, where methanol was used as a solvent. The extracted pigments were diluted in distilled water and the antimicrobial effect of the pigment was studied against Escherichia coli, Staphylococcus aureus and Salmonella typhi using agar-cup method. The clear zone of inhibition that formed around the well indicates antimicrobial activity of the pigment.