The western Himalaya is a rich repository of unique plants that are valued for their medicinal properties. Many of these plants are extensively utilized in pharmaceutical industries, and there is a huge global demand for them. Since most of these plants have become rare, threatened, or endangered, there is an urgent need to conserve them. Podophyllum hexandrum is among one of them which is an endangered medicinal plant. Hence for the regeneration and conservation of the Podophyllum hexandrum micro propagation is one of the major tools. The primary application of micro propagation has been to produce high quality planting material. However, micro propagation technology, being a capital-intensive industry is expensive. Low-cost tissue culture technology is the adoption of practices and use of equipment to reduce the unit cost of micro propagule and plant production. Low cost options should lower the cost of production without compromising the quality of the micro propagules and plants. In low cost technology cost reduction is achieved by improving process efficiency and better utilization of resources. Plants cell culture technologies were introduced at the end of 1960‘s as a possible tool for both studying and producing plant secondary metabolites. Plants secondary metabolites are unique sources for pharmaceuticals food additives, flavors and others industrial values. Commercial importance of these secondary metabolites has resulted in a great interest in its production and in exploring possibilities of enhancing its production by means of tissue culture technology.