Many plants species live in symbiotic associations with some fungal species of the glomeromycota which are popularly called as arbuscular mycorrhizal fungi (AMF). AMF helps plants to capture macro and micronutrients which result to better plant growth and performance. Investigations were carried out to ascertain the effect of bio-fertilizer on maize growth as compared to organic and inorganic fertilizers. Experiments were conducted in the field and treatments laid out in a complete randomized block design. The treatments were replicated four times each. The result of the investigations on chlorophyll a revealed that plant leaves treated with organic manure had the highest (0.955mg/g) chlorophyll a, followed by leaves of plant treated with arbuscular mycorrhizal fungi (0.84mg/g), control (0.83mg/g) leaves and lowest (0.63mg/g) value obtained from leaves fertilized with NPK fertilizer. Results revealed that leaves of maize inoculated with AMF, organic manure and untreated check were statistically (P<0.05) the similar in the chlorophyll a and were significantly different (P>0.05) from plant leaves that have been fertilized with NPK fertilizer. Although there were no significant differences (P<0.05) between the AMF, cow dung and control treatments with regard to their level of chlorophyll b, there was significant difference between cow dung manure and NPK treated plants. Result of the study in respect to roots colonization affected by NPK, cow dung manure and control were statistically (P<0.05) similar, and AMF treated plant had the highest percentage (70%). Findings from these experiments indicate AMF could be used as soil amendment since it improved crop leaves chlorophyll and root colonization respectively. Further studies on bio-fertilizer are recommended to characterize the active spores for crop developments.