The present study included isolating and diagnosing filamentous fungi and yeasts in three months starting from 15/6/2022 to 15/9/2022 from the soils of Bay Hassan oil field in Kirkuk Governorate and the K1 oil filling station in Erbil Governorate/ Iraq. 80 isolates of filamentous fungi were obtained and distributed to 3 different genera Aspergillus, Penicillium and Rhizoctonia. The fungi A. niger, A. caespitosus, A. fumigatus, A. nidulans, A. parasiticus, A. emericella quadrilieata were identified to the species level and the fungus was A. niger was the most frequent fungus in the two isolation sites, the number of isolates in (k1) filling station reached 9 (56.25%) and 15 (23.43%) isolates in Bay Hassan field, while the fungus A. fumigatus was the least frequent (6.25%) in K1 filling station. Rhizoctonia spp. was less frequent in Bay Hassan field (1.56%), and the rest of the fungi varied in their appearance rates. The study was also Isolated62 isolation back to Eight local yeast isolates were isolated, which included eight different types of yeasts: Pichia, Saccharomyces, Geotrichum, Zygosaccharomyces, Kloeckera, kazachstania, Rhodotorula, and Candida.