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VOL. 9, ISSUE 1 (2024)
Studies on Indole Acetic Acid (IAA) production by Azotobacter spp. Isolated from soil samples
Authors
Barate D L, Mhaisane D R
Abstract
Azotobacter
is considered as important fertilizing agents that contribute to the nitrogen
availability and substitute’s chemical fertilizer. Indole acetic acid (IAA) is
the most common, naturally occurring, plant hormone of the auxin class.
Screening of Azotobacter Isolate for IAA production and optimization
studies of IAA production by isolate by observing effect of various parameter
like temperature, pH, carbon source, nitrogen source, time and L-tryptophan
concentration. In the study, the isolate A3, isolate A4 and isolate A7 showed
maximum IAA production. Thus the isolate A3, isolate A4 and isolate A7 being
beneficial in increasing crop production. Studies on optimization suggest that
IAA production maximum at 37°C (temperature) for all 3 isolates at 96 Hrs of
incubation time, at pH 2. pH 7 and pH 8 respectively. Mannitol and Sucrose found
to be the best carbon source for IAA production and the Ammonium sulphate and
urea found to be the best nitrogen source for IAA production. While at 1.5%
Tryptophan, maximum IAA production for all 3 isolates.
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Pages:11-16
How to cite this article:
Barate D L, Mhaisane D R "Studies on Indole Acetic Acid (IAA) production by <i>Azotobacter spp. </i>Isolated from soil samples". International Journal of Biology Research, Vol 9, Issue 1, 2024, Pages 11-16
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