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VOL. 9, ISSUE 1 (2024)
Optimization and strain improvement by mutation for enhanced cellulase enzyme production by Bacillus sp APCMST10 isolated from coir retting effluent
Authors
Dr. M Sugumari, S Jamila Jasmin
Abstract
The goal was to investigate the cellulase enzyme production ability of
bacterial strain isolated from coir retting effluent and identified as Bacillus sp.
on the basis of 16 S rDNA sequence homology. The effects of different
nutrient sources like carbon and nitrogen, phosphate, surfactants, metal ions
and environmental factors such as temperature and pH on cellulase production by
the predominant bacterial strain were examined. Bacillus sp APCMST10
registered maximum cellulose production when cultured in the media supplied
with carboxy methyl cellulose (40.27±1.55U/ml), ammonium nitrate (34.81±0.26
U/ml), dipotassium hydrogen phosphate (44.26±0.40U/ml), tritonx100
(45.31±1.44U/ml), zinc sulphate (46.16±1.44U/ml) at 35ºC temperature
(49.33±0.63U/ml) and pH6.0(49.33±1.95U/ml), respectively. The efficiency of the
test organisms on production of cellulase enzymes after physical and chemical
mutation was also analyzed. The mutant strains Bacillus sp APCMST10
which were registered higher cellulase production when compared with that of
wild strains.
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Pages:29-34
How to cite this article:
Dr. M Sugumari, S Jamila Jasmin "Optimization and strain improvement by mutation for enhanced cellulase enzyme production by <i>Bacillus sp</i> APCMST10 isolated from coir retting effluent". International Journal of Biology Research, Vol 9, Issue 1, 2024, Pages 29-34
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