Objective: Detection of viral hepatitis B and C, acquired immunodeficiency syndrome (AIDS), syphilis, and Co-infections among serum of blood donors in Khanaqin city.

Material and methods: The conducted investigation was happened in Khanaqin city/ Iraq on blood donors who visiting the main blood bank of diyala health department for period 1/6 to 30/7 -2024. 1500 blood samples were collected from those individuals for detection positivity of microbial infections (HCV, HBS, HIV, and Treponema palladium). Enzyme-linked immune-sorbent assay (ELISA) method used to detect positivity of HCV, HBV, HIV, and Treponema palladium microbes among blood donors. SPSS v. 23.0 and Excel 2013 programs were utilized analyze our data.

Results: Study results showed the most participants were; males (78.0%) within age group 41-50 years (32.0%), live in urban (68.0%), blood groups A (36.0%) and O (34.0%), no employed (68.0%), singles (74.0%), secondary education level (56.0%), no smoking (80.0%) and no alcohol use (86.0%). The differences among percentages of demographic features were significant (p<0.05), in exception, living and occupation features were no significant (p>0.05). Positivity of microbial infections in blood donors were as following; HCV (0.4%), HBV (1.7%), HIV (0.1%), TP (1.3%), and co-infection (HBV+TP) (0.3%). Finally, the correlations between microbial infections and most demographic features were significant (p<0.05).