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International Journal of
Biology Research
ARCHIVES
VOL. 10, ISSUE 1 (2025)
Histological development study of testis and epididymis of Awassi sheep Ovis Aries at postnatal stages
Authors
Marwa Adil Hameed, Noor Adnan Azawi, Firas Abbas Hussen
Abstract

Objective: The purpose of this study was to provide a light on the rams' testicular structure at various ages.

Materials and method: 18 rams were used (6 prepuberty age 1-5 month; 6 Sexual puberty age 6-8 month and 6 Sexual maturity age 9-12 month).

Results: In each ages, a thick capsule encased the two testis. Tubules and interstitial tissue make up each testicular lobule. Multiple testicular cords were seen in the testicular parenchyma of aged 1-3 months. By the fourth month, there were very few cords. Not spermatogenesis started until the fifth month, the tunica albuginea becomes organized into outer and interior layers. The Sertoli cell was oval in shape had a euchromatic pyramidal nucleus; in the connective tissue separating adjacent tubules. The cuboidal epithelium line the rete testis had an acidic cytoplasm and spherical nucleus. Efferent ductules connected the cords to the ductus epididymis. At the 6–8 months, the testis began to mature, spermatogenesis, and some spermatozoa could observe inside the tubules. The excurrent duct system of the testis became well-organized in 9–12 months when well-developed spermatozoa were present in the lumen of seminiferous tubules. It included rete testis channels, straight tubules, and the segment of seminiferous tubules. Simple cuboidal epithelium bordered the straight tubules. Located on the basement membrane, the spermatogonia are tiny, spherical cells with black nuclei that responded to PAS. Primary spermatocytes are larger cells with often different chromatin. Because of their rapid second meiotic division, secondary spermatocytes are uncommon. Round, pale-nucleated cells called spermatids are gathered near the seminiferous tubule's lumen. Leydig cell was polymorphic with ovoid nucleus, the epididymis lined by pseudostratified epithelium with stereocilia.

Conclusion: At 9 to 12 months of age, the testis's structure resembled that found in other ruminant species; the seminiferous tubules have a large lumen and adult sperm.

Pages:38-42
How to cite this article:
Marwa Adil Hameed, Noor Adnan Azawi, Firas Abbas Hussen "Histological development study of testis and epididymis of Awassi sheep <i>Ovis Aries</i> at postnatal stages". International Journal of Biology Research, Vol 10, Issue 1, 2025, Pages 38-42
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