Objective: The purpose of this study was to provide a light on the rams'
testicular structure at various ages.
Materials and method: 18 rams were used (6 prepuberty age 1-5 month; 6 Sexual puberty age 6-8 month and
6 Sexual maturity
age 9-12 month).
Results: In each ages, a thick capsule encased the two testis. Tubules and
interstitial tissue make up each testicular lobule. Multiple testicular cords
were seen in the testicular parenchyma of aged 1-3 months. By the fourth month,
there were very few cords. Not spermatogenesis started until the fifth month,
the tunica albuginea becomes organized into outer and interior layers. The
Sertoli cell was oval in shape had a euchromatic pyramidal nucleus; in the
connective tissue separating adjacent tubules. The cuboidal epithelium line the
rete testis had an acidic cytoplasm and spherical nucleus. Efferent ductules
connected the cords to the ductus epididymis. At the 6–8 months, the testis
began to mature, spermatogenesis, and some spermatozoa could observe inside the
tubules. The excurrent duct system of the testis became well-organized in 9–12
months when well-developed spermatozoa were present in the lumen of
seminiferous tubules. It included rete testis channels, straight tubules, and
the segment of seminiferous tubules. Simple cuboidal epithelium bordered the
straight tubules. Located on the basement membrane, the spermatogonia are tiny,
spherical cells with black nuclei that responded to PAS. Primary
spermatocytes are larger cells with often different chromatin. Because of their
rapid second meiotic division, secondary spermatocytes are uncommon. Round,
pale-nucleated cells called spermatids are gathered near the seminiferous
tubule's lumen. Leydig cell was polymorphic with ovoid nucleus, the epididymis
lined by pseudostratified epithelium with stereocilia.
Conclusion: At 9 to 12 months of age, the testis's structure resembled that found
in other ruminant species; the seminiferous tubules have a large lumen and
adult sperm.
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