Macrofungi are essential components of terrestrial ecosystems, contributing significantly to nutrient cycling, decomposition, and symbiotic interactions. The present study was undertaken to document and identify          macrofungal diversity within a semi-natural college campus ecosystem in Mysuru, India. Field surveys were conducted during the monsoon and post-monsoon seasons across diverse microhabitats, including lawns, garden soils, decaying wood, tree bases, and termite mounds. Specimens were collected, photographed in situ, and identified based on macromorphological and microscopic characteristics using standard taxonomic keys. The study recorded the occurrence of five ecologically important genera: Ganoderma, Scleroderma, Leucocoprinus, Coprinellus disseminatus, and Termitomyces. Ganoderma species were predominantly associated with decaying wood and functioned as white-rot decomposers. Scleroderma species exhibited ectomycorrhizal associations with tree roots, indicating their role in plant nutrition and soil health. Leucocoprinus and Coprinellus disseminatus were observed on organic-rich substrates, contributing to rapid decomposition and nutrient recycling. Termitomyces species were closely associated with termite mounds, reflecting their obligate symbiotic relationship and role in lignocellulosic degradation. The findings highlight that even managed campus environments can support diverse and functionally significant macrofungal communities. The seasonal occurrence of these fungi during periods of high humidity underscores the influence of environmental factors on fungal growth. This study emphasizes the ecological importance of macro fungi and the need for their continued documentation and conservation in semi-urban landscapes, while also recognizing their potential ecological, economic, and biotechnological significance.