The accumulation of free radicals in the body is implicated in the development of various diseases due to oxidative stress. Current research endeavours are focused on preventing oxidative stress and mitigating the impact of free radicals through the use of antioxidants, primarily derived from plants rather than synthetic sources. In this study, Acorus calamus, a plant belonging to the Acoraceae family, was utilized to evaluate its phytochemical constituents and its ability to scavenge free radicals. To extract the Acorus calamus rhizome, four solvents—methanol, ethanol, chloroform, and water were employed. Preliminary phytochemical analysis was conducted using standard protocols. Additionally, the study assessed the free radical scavenging activity against DPPH, ABTS, FRAP, and nitric oxide. The results of the phytochemical screening indicated the presence of compounds such as alkaloids, flavonoids, sterols, proteins, and carbohydrates, with ethanol extract exhibiting higher concentrations compared to other solvents. The free radical scavenging activity demonstrated maximum efficacy in the ethanol extract, with a scavenging power of 89.53% for DPPH and 87.71% for ABTS, 88.24% for FRAP and 88.53% for Nitric oxide at a concentration of 100 μg/ml. These findings suggest that Acorus calamus rhizome has the potential to effectively scavenge free radicals, offering a promising avenue for preventing diseases associated with oxidative stress. This highlights the plant's potential as a valuable phytotherapeutic agent against a range of diseases and disorders.